Nicholson M L, Voris B P, Young D A
Cancer Res. 1981 Sep;41(9 Pt 1):3530-7.
Two-dimensional gel electrophoresis was used to detect [35S]methionine-labeled newly synthesized proteins from corticosteroid-sensitive and corticosteroid-resistant P1798 mouse lymphosarcoma cells. When the protein patterns from corticosteroid-sensitive cells are compared with those from highly resistant cells, they are very similar except for seven changes. One of the most obvious changes is the appearance of a new protein with a molecular weight of about 36,000 in highly resistant cells. There are also four other proteins that increase in the resistant state, as well as two that decrease. The control experiments indicate that protein differences are present to approximately the same degree when the sensitive tumor is carried in adrenalectomized animals, suggesting that the protein differences are not induced by endogenous glucocorticoids. Other experiments show that the protein differences are neither components of the small amounts of fibrous tissue that are associated with the cells nor components of blood. These results suggest that resistance of the tumor cells to glucocorticoid killing in vivo could be related to the relative rates of synthesis of a few proteins.
二维凝胶电泳用于检测来自皮质类固醇敏感和皮质类固醇耐药的P1798小鼠淋巴肉瘤细胞的[35S]甲硫氨酸标记的新合成蛋白质。当将皮质类固醇敏感细胞的蛋白质模式与高度耐药细胞的蛋白质模式进行比较时，除了七个变化外，它们非常相似。最明显的变化之一是在高度耐药细胞中出现了一种分子量约为36,000的新蛋白质。还有其他四种蛋白质在耐药状态下增加，以及两种减少。对照实验表明，当在肾上腺切除的动物中携带敏感肿瘤时，蛋白质差异以大致相同的程度存在，这表明蛋白质差异不是由内源性糖皮质激素诱导的。其他实验表明，蛋白质差异既不是与细胞相关的少量纤维组织的成分，也不是血液的成分。这些结果表明，肿瘤细胞在体内对糖皮质激素杀伤的抗性可能与少数蛋白质的相对合成速率有关。
