克隆的鸭β-珠蛋白cDNA的核苷酸序列分析

Nucleotide sequence analysis of a cloned duck beta-globin cDNA.

作者信息

Hampe A, Therwath A, Soriano P, Galibert F

出版信息

Gene. 1981 Jun-Jul;14(1-2):11-21. doi: 10.1016/0378-1119(81)90144-x.

Abstract

The primary structure of the duck beta-globin mRNA was obtained from sequence analysis of the double-stranded in vitro-transcribed DNA cloned in plasma pBR322. The 646-bp long globin DNA insert comprises a coding sequence of 438 bp corresponding to 146 amino acids, a 5'-noncoding region 63 bp long, and a 3'-noncoding region of 113 bp prior to a stretch of adenosine residues. The salient features of each of these regions are discussed and compared with beta-globin mRNAs of other vertebrates.

摘要

鸭β-珠蛋白mRNA的一级结构是通过对克隆于质粒pBR322中的双链体外转录DNA进行序列分析获得的。646bp长的珠蛋白DNA插入片段包含一个438bp的编码序列，对应146个氨基酸，一个63bp长的5'-非编码区，以及一段腺苷残基之前113bp的3'-非编码区。对这些区域的显著特征进行了讨论，并与其他脊椎动物的β-珠蛋白mRNA进行了比较。