鲤鱼（Cyprinus carpio）α-珠蛋白mRNA的cDNA克隆及序列分析

Cloning and sequence analysis of a cDNA for the alpha-globin mRNA of carp, Cyprinus carpio.

作者信息

Takeshita S, Aoki T, Fukumaki Y, Takagi Y

出版信息

Biochim Biophys Acta. 1984 Dec 14;783(3):265-71. doi: 10.1016/0167-4781(84)90037-x.

Abstract

A cDNA for alpha-globin mRNA of the carp, Cyprinus carpio, was cloned by the method of Okayama and Berg (Mol. Cell. Biol. 2 (1982) 161-170) and its complete nucleotide sequence was determined. The 5' non-coding region contained 23 nucleotides. Following this region, there was an open reading frame encoded with an alpha-globin polypeptide consisting of 142 amino acids. The 3' non-coding region was 88 nucleotides in length, including two copies of the hexanucleotide AATAAA and a poly(A) site of the GC dinucleotide. There were 16 discrepancies between the reported amino acid sequence of the carp alpha-globin chain and the amino acid sequence predicted from the DNA sequence of the clone. The possible explanations for these differences in amino acid sequence are discussed.

摘要

采用冈山县和伯格的方法（《分子与细胞生物学》2 (1982) 161 - 170）克隆了鲤鱼（Cyprinus carpio）α-珠蛋白mRNA的cDNA，并测定了其完整的核苷酸序列。5'非编码区包含23个核苷酸。在该区域之后，有一个开放阅读框，编码一个由142个氨基酸组成的α-珠蛋白多肽。3'非编码区长88个核苷酸，包括两个六核苷酸AATAAA拷贝和一个GC二核苷酸的多聚腺苷酸化位点。报道的鲤鱼α-珠蛋白链氨基酸序列与从克隆的DNA序列预测的氨基酸序列之间存在16个差异。讨论了这些氨基酸序列差异的可能原因。