鸡α-珠蛋白cDNA的完整核苷酸序列

Complete nucleotide sequence of a chicken alpha-globin cDNA.

作者信息

Liu A Y, Salser W

出版信息

Gene. 1981 May;13(4):409-15. doi: 10.1016/0378-1119(81)90020-2.

Abstract

The entire sequence of a 541 bp insert in recombinant plasmid pHb1003 has been determined. This plasmid, which was shown to carry a cloned cDNA copy of the chicken alpha-globin mRNA, contains the complete structural gene as well as 19 bp of the 5'-untranslated region and 99 bp of the 3'-untranslated region. This sequence may encode a non-adult alpha-globin gene, especially since the cDNA clones were generated from phenylhydrazine-induced, globin-specific mRNA extracted from anemic white leghorns. The possibility that this alpha-globin might represent a stress globin is considered.

摘要

已确定重组质粒pHb1003中541 bp插入片段的完整序列。该质粒携带鸡α-珠蛋白mRNA的克隆cDNA拷贝，包含完整的结构基因以及5′非翻译区的19 bp和3′非翻译区的99 bp。该序列可能编码一个非成人α-珠蛋白基因，特别是因为cDNA克隆是从苯肼诱导的、从贫血白来航鸡中提取的珠蛋白特异性mRNA产生的。人们考虑了这种α-珠蛋白可能代表应激珠蛋白的可能性。