Puchkov V F, Popov V B, Elinek R, Dostal M
Arkh Anat Gistol Embriol. 1981 May;80(5):104-10.
A comparative investigation on efficiency of four modern methods for testing embryotoxicity (intraamniotic administration, cultivation of postimplanted rat embryos in medium when certain teratogens were administered by various methods, test techniques on pregnant animals, testing, by CHEST--chick embryo toxicity screening test--method on chick embryos) was performed with a well known teratogen -- cyclophosphamide. A satisfactory correlation between the results was obtained, but a greater efficiency, less time for obtaining final results and greater sensitivity was noted for the new test methods in comparison to routine testing on pregnant animals. Advantages and disadvantages of every method applied were discussed.
使用一种知名致畸剂——环磷酰胺，对四种现代胚胎毒性测试方法（羊膜腔内给药、在培养基中培养植入后大鼠胚胎并通过各种方法给予某些致畸剂、对怀孕动物的测试技术、通过CHEST——鸡胚毒性筛选试验——对鸡胚的测试方法）的效率进行了比较研究。结果之间获得了令人满意的相关性，但与对怀孕动物的常规测试相比，新测试方法具有更高的效率、更短的获得最终结果的时间和更高的灵敏度。讨论了所应用的每种方法的优缺点。
