Jelínek R, Marhan O
Institute of Histology and Embryology, 3rd Medical Faculty, Charles University, Praha, Czech Republic.
Funct Dev Morphol. 1994;4(4):317-23.
With the aim of investigating the predictive value of the Chick Embryotoxicity Screening Test (CHEST) with respect to regulatory rat-rabbit procedures, we compared the results of testing 50 chemicals of different pharmacological properties. Besides all being tested in the alternative technique, 27 substances were tested both in rats and rabbits, 22 only in the rat, and one only in the rabbit, respectively. The predictive value of CHEST with respect to the both official whole-animal procedures was found satisfactory, exhibiting about 80% consistent results. It is argued that the purpose of alternative testing methods is not in replacing the official routine procedures, but in contributing to the development of new predictive systems based on recent teratological knowledge.
为了研究鸡胚毒性筛选试验（CHEST）相对于标准大鼠-兔试验程序的预测价值，我们比较了对50种具有不同药理特性的化学物质的测试结果。除了全部在替代技术中进行测试外，分别有27种物质在大鼠和兔子中都进行了测试，22种仅在大鼠中测试，1种仅在兔子中测试。发现CHEST相对于两种官方全动物试验程序的预测价值令人满意，结果一致性约为80%。有人认为，替代测试方法的目的不是取代官方常规程序，而是基于最新的致畸学知识，为新的预测系统的开发做出贡献。
