Babich A, Nevins J R
Cell. 1981 Nov;26(3 Pt 1):371-9. doi: 10.1016/0092-8674(81)90206-3.
H5ts125, a temperature-sensitive mutant of adenovirus type 5, is restricted to the early phase of infection when grown at the nonpermissive temperature. One phenotype of the virus is the overproduction of early viral mRNA at the nonpermissive temperature relative to levels found in wild-type-infected cells, although normal levels are found at the permissive temperature. We have analyzed this phenomenon for the production of RNA from two specific early viral transcription units, E1A and E1B. Transcription rates from both of these regions were found to be the same in ts125-infected cells as in wild-type-infected cells at the nonpermissive temperature. However, when the cytoplasmic stabilities of the E1A and E1B mRNAs were measured in wild-type- and ts125-infected cells, it was found that at the nonpermissive temperature, the RNAs were 3 to 5 times more stable in a ts125 infection than in a wild-type infection. Since the mutation in ts125 maps to the gene for the 72 kd DNA-binding protein, these results imply that a functional 72 kd protein is required for the rapid turnover of early viral mRNA in wild-type-infected cells, indicating that the abundance of early viral mRNA is controlled by the 72 kd DNA-binding protein.
H5ts125是5型腺病毒的一种温度敏感突变体，在非允许温度下生长时，它被限制在感染的早期阶段。该病毒的一个表型是在非允许温度下相对于野生型感染细胞中发现的水平，早期病毒mRNA过量产生，尽管在允许温度下发现的是正常水平。我们已经针对从两个特定的早期病毒转录单位E1A和E1B产生RNA的这种现象进行了分析。发现在非允许温度下，ts125感染细胞中这两个区域的转录速率与野生型感染细胞中的相同。然而，当在野生型和ts125感染细胞中测量E1A和E1B mRNA的细胞质稳定性时，发现在非允许温度下，ts125感染中的RNA稳定性比野生型感染中的高3至5倍。由于ts125中的突变定位在72kd DNA结合蛋白的基因上，这些结果表明野生型感染细胞中早期病毒mRNA的快速周转需要功能性的72kd蛋白，这表明早期病毒mRNA的丰度受72kd DNA结合蛋白控制。
