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来自不同链霉菌菌株的木聚糖水解酶及其在木聚糖分解过程中的相互影响的研究。

Studies on xylan hydrolases from different strains of Streptomyces and their mutual influences in the breakdown of xylan.

作者信息

Sreenath H K, Joseph R, Murthy V S

出版信息

Folia Microbiol (Praha). 1978;23(4):299-303. doi: 10.1007/BF02876684.

DOI:10.1007/BF02876684
PMID:689574
Abstract

A good proportion of Sreptomyces isolates from natural sources produced extracellular xylan hydrolase. Nineteen isolated showing high activity were able to completely or partially degrade wheat bran in the growth medium. Chromatographic analysis of commercial xylan degradation products suggested that the isolates produced either endo- or exo-xylan hydrolases or their mixtures. Mixed additions of culture fluids showed a highly synergistic effect, up to an increase by 200%. In a few cases antagonism was seen which, however, could be removed by dialysis of the culture fluid.

摘要

从天然来源分离出的链霉菌中有相当一部分能产生胞外木聚糖水解酶。19株显示出高活性的菌株能够在生长培养基中完全或部分降解麦麸。对商业木聚糖降解产物的色谱分析表明,这些菌株产生的要么是内切木聚糖水解酶,要么是外切木聚糖水解酶,或者是它们的混合物。混合添加培养液显示出高度的协同效应,增幅高达200%。在少数情况下观察到拮抗作用,不过,通过对培养液进行透析可以消除这种拮抗作用。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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弗氏链霉菌生产木糖异构酶的培养稳定性:摇瓶研究
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Chemical modification of a xylanase from a thermotolerant Streptomyces. Evidence for essential tryptophan and cysteine residues at the active site.来自耐热链霉菌的木聚糖酶的化学修饰。活性位点上必需色氨酸和半胱氨酸残基的证据。
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