利用来自健康番茄叶片组织的依赖RNA的RNA聚合酶，将类病毒RNA体外转录为全长拷贝。

In vitro transcription of viroid RNA into full-length copies by RNA-dependent RNA polymerase from healthy tomato leaf tissue.

作者信息

Boege F, Rohde W, Sänger H L

出版信息

Biosci Rep. 1982 Mar;2(3):185-94. doi: 10.1007/BF01116382.

Abstract

RNA-dependent RNA polymerase from healthy tomato plant tissue accepts potato spindle tuber viroid (PSTV) RNA as a template for the in vitro synthesis of full-length RNA copies of the PSTV genome. Viroid transcription requires the presence of Mn2+ and /or Mg2+ ions and is not inhibited by concentrations of 10(-5) M alpha-amanitin. This is the first report of a well-defined product synthesized in vitro by an RNA-dependent RNA polymerase from healthy plants.

摘要

来自健康番茄植株组织的RNA依赖性RNA聚合酶能够接受马铃薯纺锤块茎类病毒（PSTV）RNA作为模板，用于体外合成PSTV基因组的全长RNA拷贝。类病毒转录需要存在Mn2+和/或Mg2+离子，并且不受10（-5）M浓度的α-鹅膏蕈碱的抑制。这是关于健康植物来源的RNA依赖性RNA聚合酶在体外合成明确产物的首次报道。