Skul'skiĭ I A, Glazunov V V
Tsitologiia. 1982 Feb;24(2):183-7.
Unidirectional fluxes of tracers 137Cs+ (+valinomycin) and 204Tl+ (+nonactin) in mitochondria maintained under stationary state at 0 degree C have been studied. The tracer uptake in energized mitochondria (Q infinity Cmit/Cmed = Kin/kout) was about 10 times as high compared to that in a deenergized state. Energization caused a 5-10 fold increases of the influx rate constant kin, while the efflux rate constant, kout, decreased only by 1.5-2 times of less. There was a positive correlation between Q infinity and kin. No correlation could be found between Q infinity and kout. The results obtained suggest that under the experimental conditions studied the energization did not increase substantially the membrane potential expected to retard the efflux of permeant cations. In the presence of ionophores the monovalent cations are supposed to be accumulated in mitochondria against the electrochemical gradient.
研究了在0℃下处于稳态的线粒体中示踪剂137Cs+（+缬氨霉素）和204Tl+（+尼日利亚菌素）的单向通量。与去能状态相比，在有能量的线粒体中示踪剂的摄取量（Q∞=Cmit/Cmed=Kin/kout）约高10倍。能量化使流入速率常数kin增加了5-10倍，而流出速率常数kout仅降低了1.5-2倍。Q∞与kin之间存在正相关。在Q∞与kout之间未发现相关性。所获得的结果表明，在所研究的实验条件下，能量化并没有实质性地增加预期会阻碍渗透性阳离子流出的膜电位。在离子载体存在的情况下，单价阳离子被认为是逆着电化学梯度在线粒体中积累的。
