Sukl'skiĭ I A, Glazunov V V, Ivanova T I, Savina M V, Baklanova S M
Zh Evol Biokhim Fiziol. 1984 May-Jun;20(3):246-50.
Studies have been made on the valinomycin-induced transport of Cs+ at 0 degree C in rat liver mitochondria energized with succinate. Under these conditions, mitochondria preserve their ability to accumulate Cs+ for 6 hours of incubation or even longer. The rate of both the inward and outward movement of Cs+ at 0 degree C was approximately an order lower than at room temperature, although the stationary distribution of Cs+ remained at a high level. Electron microscopic observations revealed no significant changes in the structure of mitochondria in spite of their prolonged incubation. These properties of mitochondria make it possible to determine the kinetic parameters of ion transport and in this way to evaluate functional condition of mitochondria at low temperatures.
已经对缬氨霉素在0℃下诱导琥珀酸供能的大鼠肝线粒体中Cs+的转运进行了研究。在这些条件下，线粒体在孵育6小时甚至更长时间内保持其积累Cs+的能力。尽管Cs+的稳态分布仍处于高水平，但在0℃下Cs+向内和向外移动的速率大约比室温下低一个数量级。电子显微镜观察表明，尽管线粒体长时间孵育，但其结构没有明显变化。线粒体的这些特性使得确定离子转运的动力学参数成为可能，从而评估线粒体在低温下的功能状态。
