Thim L, Jørgensen K H, Jørgensen K D
Regul Pept. 1982 Mar;3(3-4):221-30. doi: 10.1016/0167-0115(82)90127-6.
A radioimmunoassay (RIA) for the determination of porcine Pancreatic Spasmolytic Polypeptide (PSP) has been developed. The antisera raised in rabbits were sensitive to 5 pgequiv. of PSP in a volume of 100 microliter. Immunoreactive PSP (IR-PSP) has been determined in extracts from 22 porcine organs. Pancreas was found to be the only organ containing substantial amounts of IR-PSP (0.1 mgequiv. IR-PSP/g wet weight). The fasting porcine plasma level of IR-PSP was about 10 ngequiv./ml, corresponding to 850 pM. The concentration of IR-PSP in porcine pancreatic juice varied from 0.2 mugequiv./ml in the fasting state to 46-116 mugequiv./ml after stimulation with pancreozymin or secretin. A linear correlation was found between the exocrine secretion of IR-PSP and total protein.
已开发出一种用于测定猪胰腺解痉多肽（PSP）的放射免疫分析法（RIA）。在兔体内产生的抗血清对100微升体积中5皮克当量的PSP敏感。已测定了22个猪器官提取物中的免疫反应性PSP（IR-PSP）。发现胰腺是唯一含有大量IR-PSP的器官（0.1毫克当量IR-PSP/克湿重）。空腹猪血浆中IR-PSP的水平约为10纳克当量/毫升，相当于850皮摩尔。猪胰液中IR-PSP的浓度在空腹状态下为0.2微克当量/毫升，在用促胰酶素或促胰液素刺激后为46 - 116微克当量/毫升。发现IR-PSP的外分泌与总蛋白之间存在线性相关性。
