Phelps B M, Williamson P, Schlegel R A
J Cell Physiol. 1982 Mar;110(3):245-8. doi: 10.1002/jcp.1041100304.
Experiments were carried out to examine the possible physiological role of disordered membrane domains in hematopoietic cell surface differentiation. The hematopoietic stem cell line 416B has been shown to bind the dye merocyanine 540 (MC540), a fluorescent probe which may be specific for disordered regions of lipid bilayers (Williamson et al., 1981). The surface receptors for the lectins Concanavalin A (Con A) and wheat germ agglutinin (WGA) exhibit patchy distributions on the surface of 416B cells which correspond to the distribution of MC540 binding regions. Appropriate incubation of these cells with either of the two lectins results in the induced formation of a cap. The binding regions for MC540 and the receptors for the other lectin become localized to the same region of the membrane by this process. Such coordinated rearrangements of surface glycoproteins associated with disordered lipid domains may play a role in the cocapping of disparate surface molecules (Raz and Bucana, 1980) or in differentiation-related cell surface rearrangements (Schlegel et al., 1980).
开展了实验以研究无序膜结构域在造血细胞表面分化中可能的生理作用。造血干细胞系416B已被证明能结合染料部花青540（MC540），这是一种荧光探针，可能对脂质双层的无序区域具有特异性（威廉姆森等人，1981年）。凝集素刀豆球蛋白A（Con A）和麦胚凝集素（WGA）的表面受体在416B细胞表面呈现斑驳分布，这与MC540结合区域的分布相对应。用这两种凝集素中的任何一种对这些细胞进行适当孵育会导致帽状结构的诱导形成。通过这个过程，MC540的结合区域和另一种凝集素的受体定位于膜的同一区域。与无序脂质结构域相关的表面糖蛋白的这种协同重排可能在不同表面分子的共帽形成（拉兹和布卡纳，1980年）或与分化相关的细胞表面重排（施莱格尔等人，1980年）中发挥作用。
