Raleigh J A, Liu S F, Shum F Y
Int J Radiat Oncol Biol Phys. 1982 Mar-Apr;8(3-4):701-4. doi: 10.1016/0360-3016(82)90716-7.
Reductive activation of misonidazole and misondiazole acetate, a simple derivative, in the presence of xanthine oxidase causes inactivation of the enzyme. The inactivation is not accompanied by binding of the misonidazole to the enzyme. The nitroreductase activity of xanthine oxidase is inhibited as measured by the reduction of 3,5-dinitrobenzonitrile (DNBN). Cysteine does not appear to protect against the enzyme inactivation by misonidazole but, by itself, cysteine has a strong stimulating effect on the reduction of DNBN. The possible significance of these reactions to the toxicity of misonidazole are discussed.
在黄嘌呤氧化酶存在的情况下，甲硝唑和一种简单衍生物醋酸米索硝唑的还原激活会导致该酶失活。这种失活并不伴随着甲硝唑与酶的结合。通过3,5 - 二硝基苯甲腈（DNBN）的还原测定，黄嘌呤氧化酶的硝基还原酶活性受到抑制。半胱氨酸似乎不能防止甲硝唑对酶的失活作用，但半胱氨酸本身对DNBN的还原有很强的刺激作用。讨论了这些反应对甲硝唑毒性的可能意义。
