Roos E, Van de Pavert I V
J Cell Sci. 1982 Jun;55:233-45. doi: 10.1242/jcs.55.1.233.
The influence of tubulin-binding agents on the infiltration of murine MB6A lymphosarcoma and TA3 mammary carcinoma cells into primary rat hepatocyte cultures was studied. Colchicine, nocodazole and vinblastine reduced the number of infiltrating lymphosarcoma cells, probably by interfering with the adhesion of these cells to the exposed hepatocyte surface. However, the subsequent infiltration of cells that did adhere was not affected or even slightly stimulated. The reduced adhesion appears to be due to an effect on both the MB6A cells and the hepatocytes. In contrasts, adhesion of TA3 cells was not reduced and infiltration was markedly enhanced by these agents, due to an effect on the TA3 cells but probably not on the hepatocytes. These observations support previously described morphological evidence for differences between the infiltration mechanisms of the two tumour cell types. It is concluded that the system within the hepatocytes involved in adhesiveness of the exposed surface to MB6A cells is distinct from that mediating other types of adhesion. The tendency of TA3 cells to invaginate hepatocytes may be due to disturbances in tubulin-dependent processes.
研究了微管蛋白结合剂对小鼠MB6A淋巴肉瘤细胞和TA3乳腺癌细胞浸润原代大鼠肝细胞培养物的影响。秋水仙碱、诺考达唑和长春碱减少了浸润性淋巴肉瘤细胞的数量，可能是通过干扰这些细胞与暴露的肝细胞表面的黏附。然而，随后黏附的细胞的浸润并未受到影响，甚至略有增强。黏附减少似乎是由于对MB6A细胞和肝细胞都有影响。相比之下，这些药物并未降低TA3细胞的黏附，反而显著增强了其浸润，这是由于对TA3细胞有影响，但可能对肝细胞没有影响。这些观察结果支持了先前描述的两种肿瘤细胞类型浸润机制差异的形态学证据。得出的结论是，肝细胞内参与暴露表面与MB6A细胞黏附的系统与介导其他类型黏附的系统不同。TA3细胞使肝细胞内陷的倾向可能是由于微管蛋白依赖性过程的紊乱。
