Borsuk E
J Embryol Exp Morphol. 1982 Jun;69:215-22.
Diploid gynogenetic mouse embryos were produced in a three-step procedure: fertilization in vitro, suppression of the 2nd polar body formation by Cytochalasin B, and microsurgical removal of the male pronucleus. The operated eggs were transplanted to the oviduct of recipient females for 72 or 96 h. The overall recovery rate was 73%, but compacted morulae and blastocysts constituted only 28.6% of transplanted eggs. After 72 h blastocysts were rare (3.5%) but 24 h later their incidence increased to 21.2%. In eggs homozygous for T6 chromosome it was possible to prove karyologically that the male pronucleus was effectively removed and that the diploid genome was of purely maternal origin.
体外受精、用细胞松弛素B抑制第二极体形成以及显微手术去除雄原核。将操作后的卵移植到受体雌性的输卵管中72或96小时。总体回收率为73%，但致密桑椹胚和囊胚仅占移植卵的28.6%。72小时后囊胚很少见（3.5%），但24小时后其发生率增加到21.2%。在T6染色体纯合的卵中，从核型上可以证明雄原核被有效去除，并且二倍体基因组完全来自母方。
