Rowe E S
Mol Pharmacol. 1982 Jul;22(1):133-9.
The specific effect of ethanol on several aspects of the gel-to-liquid crystal transition of dipalmitoylphosphatidylcholine was investigated using two spectrophotometric techniques, one probe method and one direct method. Ethanol shifts the phase-transition temperature to low temperature, demonstrating that ethanol interacts preferentially with the fluid phase. Thermodynamic analysis of the melting point depression leads to a calculated membrane:buffer partition coefficient of 6.25 (mole fraction units) or 0.15 mole of ethanol per kilogram of lipid:mole of ethanol per liter of solution. Careful evaluation of the transition cooperativity with temperature resolution of +/- 0.1 degrees shows that there is no reduction in transition cooperativity, and thus no reduction in size of the cooperative lipid clusters due to ethanol. The implications of these findings for the mechanism of action of ethanol in terms of current theories of anesthetic mechanisms are discussed.
使用两种分光光度技术，即一种探针法和一种直接法，研究了乙醇对二棕榈酰磷脂酰胆碱凝胶 - 液晶转变几个方面的具体影响。乙醇将相转变温度移至低温，表明乙醇优先与液相相互作用。对熔点降低进行热力学分析得出计算的膜：缓冲液分配系数为6.25（摩尔分数单位）或每千克脂质0.15摩尔乙醇：每升溶液中的乙醇摩尔数。以±0.1摄氏度的温度分辨率仔细评估转变协同性表明，由于乙醇，转变协同性没有降低，因此协同脂质簇的大小也没有减小。根据当前麻醉机制理论讨论了这些发现对乙醇作用机制的意义。
