二棕榈酰磷脂酰胆碱囊泡在4摄氏度下的融合

Fusion of dipalmitoylphosphatidylcholine vesicles at 4 degrees C.

作者信息

Wong M, Anthony F H, Tillack T W, Thompson T E

出版信息

Biochemistry. 1982 Aug 17;21(17):4126-32. doi: 10.1021/bi00260a032.

Abstract

Small sonicated dipalmitoylphosphatidylcholine vesicles when incubated at 4 degrees C and high concentrations are shown to fuse completely to vesicles about 700-A diameter in 7 days, and these further fuse to about 950 A diameter vesicles after 3-4 weeks. The 950 A diameter vesicles are spherical, homogeneous, mostly unilamellar, have an internal aqueous space about 10 times that of small vesicles, and are stable for at least 6 months. The 950-A vesicles are characterized by agarose gel chromatography, freeze-fracturing electron microscopy, trapped volume measurements, differential scanning calorimetry, and diphenylhexatriene fluorescence polarization.

摘要

当在4摄氏度和高浓度下孵育时，小的经超声处理的二棕榈酰磷脂酰胆碱囊泡在7天内被证明能与直径约700埃的囊泡完全融合，并且在3 - 4周后这些囊泡进一步融合成直径约950埃的囊泡。直径950埃的囊泡呈球形、均匀，大多为单层，其内部水相空间约为小囊泡的10倍，并且至少6个月内稳定。通过琼脂糖凝胶色谱法、冷冻断裂电子显微镜、捕获体积测量、差示扫描量热法和二苯基己三烯荧光偏振对950埃的囊泡进行了表征。