二棕榈酰磷脂酰胆碱囊泡的融合

Fusion of dipalmitoylphosphatidylcholine vesicles.

作者信息

Schullery S E, Schmidt C F, Felgner P, Tillack T W, Thompson T E

出版信息

Biochemistry. 1980 Aug 19;19(17):3919-23. doi: 10.1021/bi00558a005.

Abstract

Small unilamellar dipalmitoylphosphatidylcholine vesicles formed by sonication are shown to fuse spontaneously below the phase transition temperature. The ultimate fusion products are unilamellar vesicles about 700 A in diameter, which are stable and provide an intact ionic permeation barrier either above or below the phase transition. The fused vesicles have been characterized by gel chromatography, trapped volume, 31P nuclear magnetic resonance, and negative stain and freeze-fracture electron microscopy.

摘要

通过超声处理形成的小单层二棕榈酰磷脂酰胆碱囊泡在相变温度以下会自发融合。最终的融合产物是直径约700埃的单层囊泡，它们很稳定，在相变温度以上或以下都能提供完整的离子渗透屏障。已通过凝胶色谱法、截留体积、31P核磁共振以及负染色和冷冻断裂电子显微镜对融合囊泡进行了表征。