Kajita Y, Ishida M, Hachiya T, Miyazaki T, Yoshimura M, Ijichi H, Ochi Y
Endocrinol Jpn. 1982 Apr;29(2):169-75. doi: 10.1507/endocrj1954.29.169.
An assay for detection of serum IgG binding to thyroid membrane using 125I-Protein A was examined. The test serum is incubated with purified thyroid membranes, and the IgG bound to the membrane is detected by its interaction with 125I-Protein A. Most of the bound IgG is not bound to the TSH receptor, because TSH does not induce any appreciable decrease in the binding of the IgG to thyroid membranes. Increased serum IgG binding to thyroid membrane is found in most patients with Graves' disease and Hashimoto's thyroiditis, but not in patients with thyroid cancer or simple goiter. Many sera with positive binding activity showed positive microsomal antibody. Serum IgG binding to thyroid membrane in Graves' disease correlates neither with LATS activity nor thyroglobulin antibody. This finding suggests that TSH receptor is not involved in the reaction. The assay method is useful for measuring the binding immunoglobulin for thyroid membrane that is frequently increased in autoimmune thyroid disease, and the present data provide further support to the concept that thyroid autoimmune disorders are associated with antibodies to thyroid cell surface components.
研究了一种使用¹²⁵I - 蛋白A检测血清IgG与甲状腺膜结合的检测方法。将测试血清与纯化的甲状腺膜一起孵育，通过其与¹²⁵I - 蛋白A的相互作用检测结合到膜上的IgG。大多数结合的IgG不与促甲状腺激素（TSH）受体结合，因为TSH不会导致IgG与甲状腺膜结合的明显减少。在大多数格雷夫斯病和桥本甲状腺炎患者中发现血清IgG与甲状腺膜的结合增加，但甲状腺癌或单纯性甲状腺肿患者中未发现。许多具有阳性结合活性的血清显示出阳性微粒体抗体。格雷夫斯病中血清IgG与甲状腺膜的结合既不与长效甲状腺刺激素（LATS）活性相关，也不与甲状腺球蛋白抗体相关。这一发现表明TSH受体不参与该反应。该检测方法可用于测量自身免疫性甲状腺疾病中经常增加的甲状腺膜结合免疫球蛋白，目前的数据进一步支持了甲状腺自身免疫性疾病与甲状腺细胞表面成分抗体相关的概念。
