Bates D A, Winterbourn C C
FEBS Lett. 1982 Aug 16;145(1):137-42. doi: 10.1016/0014-5793(82)81222-2.
We report our finding that the reaction between the adriamycin semiquinone (produced by reduction of the drug by xanthine oxidase) and H2O2 in N2 causes deoxyribose degradation to a thiobarbituric acid-reactive chromogen. Deoxyribose breakdown was inhibited by scavengers of hydroxyl radicals, providing evidence for the participation of hydroxyl radicals. The reaction was detected in air, but was less efficient in air than in N2. Deoxyribose degradation did not require a metal catalyst, and was inhibited by superoxide dismutase in air, but not N2. A similar reaction with deoxyribose in DNA may be of major importance in the antitumour action of adriamycin.
我们报告了一项研究发现，即阿霉素半醌（由黄嘌呤氧化酶还原该药物产生）与氮气中的过氧化氢之间的反应会导致脱氧核糖降解为硫代巴比妥酸反应性发色团。脱氧核糖的分解受到羟自由基清除剂的抑制，这为羟自由基的参与提供了证据。该反应在空气中也能检测到，但在空气中的效率低于在氮气中。脱氧核糖的降解不需要金属催化剂，在空气中会受到超氧化物歧化酶的抑制，但在氮气中则不会。与DNA中的脱氧核糖发生的类似反应可能在阿霉素的抗肿瘤作用中具有重要意义。
