Aksenova N N, Tsikarishvili T N
Neoplasma. 1982;29(3):249-56.
The hemolytic plaque assay of Jerne and Nordin has been used for the investigation of the ability of the transplantable rat lymphosarcoma cells to synthesize immunoglobulins. The data obtained indicate that in 3-4 days after antigen (sheep red blood cells) administration to the tumor-bearing rats, antibody (hemolysin) producers appear among lymphosarcoma cells. Most representative are the experiments on rats in which their own immune response was totally suppressed by X-ray irradiation. The induced hemolysis synthesis in rat lymphosarcoma cells persists at least 9 during successive transplantations of "primary immunized" tumors (45 cell generations). Total cytoplasmic RNA isolated from spleens of sheep red blood cells immunized rats induced hemolysin synthesis in lymphosarcoma cells in vitro. Such cells being inoculated into intact rats retained their ability to synthesize hemolysin up to 2-3 passages of tumor.
耶尔恩和诺丁的溶血空斑试验已用于研究可移植大鼠淋巴肉瘤细胞合成免疫球蛋白的能力。获得的数据表明，在给荷瘤大鼠注射抗原（绵羊红细胞）后3 - 4天，淋巴肉瘤细胞中出现了抗体（溶血素）产生细胞。最具代表性的是对大鼠进行的实验，这些大鼠自身的免疫反应被X射线照射完全抑制。在“初次免疫”肿瘤的连续移植（45个细胞代）过程中，大鼠淋巴肉瘤细胞中诱导的溶血素合成至少持续9代。从绵羊红细胞免疫大鼠的脾脏中分离出的总细胞质RNA在体外诱导淋巴肉瘤细胞合成溶血素。将此类细胞接种到未受影响的大鼠体内，它们在肿瘤传代2 - 3次时仍保留合成溶血素的能力。
