Kastin A J, Banks W A, Castellanos P F, Nissen C, Coy D H
Pharmacol Biochem Behav. 1982 Dec;17(6):1187-91. doi: 10.1016/0091-3057(82)90118-6.
Delta sleep-inducing peptide (DSIP) or five closely related peptides were injected peripherally and the levels of DSIP-like immunoreactivity measured in the brains of 100 g and 500 g rats decapitated a minute later after washout with 0.9% NaCl. Higher concentrations of immunoreactive material were found in brain tissue with injection of des Trp1-DSIP, D-Ala4-DSIP, and, after correction for cross-reactivity, with D-Ala4-DSIP-NH2 than with the other peptides. Both peptides substituted in the fourth position with D-alanine resulted in higher concentrations in 500 g rats than in 100 g rats, indicating a possible effect of age. The 5-10 fold greater brain to blood ratios of radioactivity after 125I-N-Tyr-DSIP than after 125-I radioiodinated serum albumin (RISA) provided additional evidence against non-specific leakage or contamination of the brains with substantial amounts of residual blood. The results suggest that penetration of the blood-brain barrier (BBB) by small amounts of peptides, at least DSIP peptides, may be somewhat selective.
将δ睡眠诱导肽（DSIP）或五种密切相关的肽经外周注射，然后用0.9%氯化钠冲洗一分钟后，对100克和500克大鼠断头，测量其大脑中DSIP样免疫反应性水平。与其他肽相比，注射去色氨酸1-DSIP、D-丙氨酸4-DSIP以及校正交叉反应后注射D-丙氨酸4-DSIP-NH2时，脑组织中发现的免疫反应性物质浓度更高。在第四位被D-丙氨酸取代的两种肽在500克大鼠中的浓度高于100克大鼠，表明可能存在年龄效应。125I-N-酪氨酸-DSIP后放射性的脑血比是125I放射性碘化血清白蛋白（RISA）后的5-10倍，这进一步证明大脑不存在大量残留血液的非特异性渗漏或污染。结果表明，少量肽，至少是DSIP肽，穿过血脑屏障（BBB）可能具有一定的选择性。
