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A modified wire-loop method for quantitative electron microscopic radioautography.

作者信息

Yoshida K, Murata F, Ohno S, Nagata T

出版信息

Histochemistry. 1978 Aug 15;57(1):93-6. doi: 10.1007/BF00507359.

DOI:10.1007/BF00507359
PMID:689928
Abstract

A simplified method of emulsion coating and development for quantitative electron microscopic radioautography without any special instrument in introduced. Seven grids carrying ultrathin sections are attached to a glass block with double-coated Scotch tape. They are coated with a wire-loop, 2.5 cm in diameter. Ten glass blocks are then stuck on a slide, exposed, developed, fixed and stained simultaneously. Employing this method, the emulsion coating and the following procedures are easily accomplished under an identical condition.

摘要

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本文引用的文献

1
AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST.电子显微镜放射自显影术。一种提高分辨率、灵敏度和对比度的方法。
J Cell Biol. 1964 Aug;22(2):469-77. doi: 10.1083/jcb.22.2.469.
2
High-resolution autoradiography. I. Methods.高分辨率放射自显影术。I. 方法。
J Cell Biol. 1962 Nov;15(2):173-88. doi: 10.1083/jcb.15.2.173.
3
A modification of dry-mounting technique for radioautography of water-soluble compounds.水溶性化合物放射自显影干装片技术的一种改进。
Histochemie. 1966;7(4):370-1. doi: 10.1007/BF00306625.
4
A new technique for electron microscopic dry-mounting radioautography of soluble-compounds.一种用于可溶性化合物电子显微镜干式放射自显影的新技术。
Histochemie. 1969;18(3):241-9. doi: 10.1007/BF00306170.
5
A reliable, standardized method for ultrastructural electron microscopic radioautography.一种用于超微结构电子显微镜放射自显影的可靠、标准化方法。
Histochemie. 1973 Oct 3;37(1):1-17. doi: 10.1007/BF00306855.