Burry R W, Lasher R S
J Microsc. 1975 Aug;104(3):307-19. doi: 10.1111/j.1365-2818.1975.tb04029.x.
A coating technique for electron microscope autoradiography is described which combines the advantage of forming an emulsion film by a dipping method with the ease of coating sections already on grids. Sections are coated so that a formvar support film separates the section and the emulsion crystals. This intermediate layer of formvar ensures a random distribution of the emulsion crystals. Using light gold sections, Ilford L-4 emulsion and Microdol-X development, the resolution of this technique, as determined by the half distance method, was 150 nm. The additional layer of formvar slightly reduced the image quality with biological samples in the electron microscope. This technique has a minimal loss of resolution and image quality for moderate resolution electron microscope autoradiography.
本文描述了一种用于电子显微镜放射自显影的镀膜技术，该技术结合了浸涂法形成乳剂膜的优点以及在已置于网格上的切片上镀膜的便利性。切片镀膜后，一层福尔马林支持膜将切片与乳剂晶体分隔开。福尔马林这一中间层确保了乳剂晶体的随机分布。使用浅金切片、伊尔福L - 4乳剂和微粒显影剂Microdol - X，通过半距离法测定，该技术的分辨率为150纳米。福尔马林附加层在电子显微镜下对生物样品的图像质量略有降低。对于中等分辨率的电子显微镜放射自显影，该技术的分辨率和图像质量损失最小。
