Wortberg B, Woller R, Chulamorakot T
J Chromatogr. 1978 Aug 21;156(1):205-10. doi: 10.1016/s0021-9673(00)83141-6.
There are different national food regulations concerning the use of hormoneactive substances in the breeding of domestic animals that serve as human food. This situation requires specific and sensitive methods for the detection of any residues in food. The hormones used in practice can be determined with improved sensitivity by coupling to the diazonium compound Fast Dark Blue R Salt. After thin-layer chromatography on high-performance thin-layer chromatographic plates with two different solvent mixtures, zeranol, estradiol, estriol and estrone are analysed as azo dyestuffs. The detection limit of these hormones is below 10 ng.
关于在用作人类食物的家畜养殖中使用激素活性物质，存在不同的国家食品法规。这种情况需要特定且灵敏的方法来检测食品中的任何残留物。通过与重氮化合物固蓝盐R偶联，可以提高实际使用的激素的检测灵敏度。在使用两种不同溶剂混合物的高效薄层色谱板上进行薄层色谱后，将玉米赤霉醇、雌二醇、雌三醇和雌酮作为偶氮染料进行分析。这些激素的检测限低于10纳克。
