Nakao J, Chang W C, Murota S I, Orimo H
Atherosclerosis. 1980 Aug;36(4):539-44. doi: 10.1016/0021-9150(80)90246-4.
Elastinolytic activity was examined in cultured rat aortic smooth muscle cells, using Congo Red elastin as a substrate. Elastinolytic activity was demonstrated in the soluble fraction of sonicated smooth muscle cells, with an optimal pH around 10.0. The soluble fraction also showed elastase-like esterolytic activity against the synthetic substrate, N-succinyl-trialanyl-paranitroanilide.
以刚果红弹性蛋白为底物，检测培养的大鼠主动脉平滑肌细胞中的弹性蛋白酶活性。在超声处理的平滑肌细胞的可溶部分中显示出弹性蛋白酶活性，最适pH约为10.0。该可溶部分还对合成底物N-琥珀酰-丙氨酰-对硝基苯胺表现出类弹性蛋白酶酯解活性。
