Engvall E, Pihko H, Jalanko H, Ruoslahti E
J Natl Cancer Inst. 1977 Jul;59(1):277-80. doi: 10.1093/jnci/59.1.277.
Antibodies to autologous alpha-fetoprotein (AFP) were produced in mice by immunization with rat AFP. C57L/J mice with or without such antibodies were inoculated sc or ip with controlled numbers of cells of the syngeneic, AFP-producing, BW 7756 hepatoma. There was a linear relationship between circulating AFP and tumor mass, with elevated AFP being detectable earlier than the tumor. The AFP levels of the immunized mice were generally lower than those of control mice, and tumors could be detected before elevated concentrations of AFP appeared in the circulation. An extensive series of transplantations with varying protocols for immunization did not protect against tumor and did not affect the rate of tumor growth.
通过用大鼠甲胎蛋白免疫，在小鼠体内产生了针对自身甲胎蛋白（AFP）的抗体。给有或没有此类抗体的C57L/J小鼠皮下或腹腔接种同基因、产生AFP的BW 7756肝癌的可控数量细胞。循环中的AFP与肿瘤大小呈线性关系，AFP升高比肿瘤更早可检测到。免疫小鼠的AFP水平通常低于对照小鼠，并且在循环中出现AFP浓度升高之前就能检测到肿瘤。一系列采用不同免疫方案的广泛移植实验并未预防肿瘤，也未影响肿瘤生长速度。
