Mizejewski G J, Young S R, Allen R P
J Natl Cancer Inst. 1975 Jun;54(6):1361-7. doi: 10.1093/jnci/54.6.1361.
Hepatoma cells derived from The Jackson Laboratory mouse hepatoma BW7756 synthesized alpha fetoprotein (AFP) in vitro. The AFP was immunologically identical to that circulating in the sera of hepatoma-bearing mice. An in vitro cytotoxic effect of rabbit antiserum to AFP was studied in hepatoma cells obtained both from fresh cell suspensions and short-term cell culture. The use of intact and/or inactivated anti-AFP serum inhibited the growth of the AFP-producing cells. The cytotoxic effects of the antiserum depended on exposure time and serum concentration. The cytotoxicity was complement independent, as demonstrated by studies with heat-deactivated serum devoid of extrinsic complement. The control target cells included fresh cell suspensions of normal mouse liver and mouse muscle fibroblasts grown in short-term culture. Specificity of the antisera for the target cells was demonstrated by absorption with purified mouse AFP. The results could be explained by the presence of AFP on the hepatoma cell surface.
源自杰克逊实验室小鼠肝癌BW7756的肝癌细胞在体外合成甲胎蛋白（AFP）。该AFP在免疫上与荷瘤小鼠血清中循环的AFP相同。在从新鲜细胞悬液和短期细胞培养物中获得的肝癌细胞中研究了兔抗AFP血清的体外细胞毒性作用。使用完整和/或灭活的抗AFP血清可抑制产生AFP的细胞的生长。抗血清的细胞毒性作用取决于暴露时间和血清浓度。如用不含外源性补体的热灭活血清进行的研究所证明，细胞毒性是不依赖补体的。对照靶细胞包括正常小鼠肝脏的新鲜细胞悬液和短期培养中生长的小鼠肌肉成纤维细胞。通过用纯化的小鼠AFP吸收来证明抗血清对靶细胞的特异性。这些结果可以通过肝癌细胞表面存在AFP来解释。
