Gilbertson J R, Gelman R A, Chiu T H, Gilbertson L I, Knauer T E
J Lipid Res. 1978 Aug;19(6):757-62.
Primary alcohols occur as trace lipids and are the only long-chain alcohol species present in Clostridium butyricum. Secondary alcohols do not occur physiologically in this microorganism. Exposure of these cells to the methyl ketone, pentadecan-2-one, results in a marked decrease in the primary alcohol content with the secondary alcohol, pentadadecan-2-ol, becoming the major alcohol present. This change in lipid composition is associated with a significant decrease in growth rate that is proportional to the log of the pentadecan 2-one concentration of the incubation medium. When these cells are incubated with pentadecan-2-ol alone, growth is unaffected. Simultaneous exposure of the bacteria to pentadecan-2-one and a mixture of primary alcohols results in a partial relief of the growth inhibition observed with the ketone alone. These observations indicate that pentadecan-2-one inhibits the formation of primary alcohols that are important for normal growth of this bacterium.
伯醇以痕量脂质形式存在，是丁酸梭菌中唯一存在的长链醇类物质。仲醇在这种微生物中不会生理存在。将这些细胞暴露于甲基酮十五烷-2-酮中，会导致伯醇含量显著降低，仲醇十五烷-2-醇成为主要存在的醇类。脂质组成的这种变化与生长速率的显著降低有关，生长速率的降低与孵育培养基中十五烷-2-酮浓度的对数成正比。当这些细胞仅与十五烷-2-醇一起孵育时，生长不受影响。细菌同时暴露于十五烷-2-酮和伯醇混合物中，可部分缓解单独使用酮时观察到的生长抑制。这些观察结果表明，十五烷-2-酮抑制了对该细菌正常生长重要的伯醇的形成。
