Gilbertson J R, Fletcher R D, Kawalek J C, Demcisak B
Lipids. 1976 Mar;11(3):172-8. doi: 10.1007/BF02532854.
The effect of inclusion of trace amounts of pentadecan-2-one in the incubation medium on the growth of HeLa Cells was evaluated by measuring viable cell counts (cells excluding trypan blue) and incorporation of [14C] leucine into acid precipitable protein. Evidence is presented to show that exposure of the cells to trace amounts of the methyl ketone, 36mug/ml, effectively inhibits cell growth. This inhibition is relieved by simultaneously incubating the cells with a long chain primary alcohol, hexadecan-1- o1, but not with the secondary alcohol, pentadecan-2-o1. The observation that the ethyl ketone, hexadecan-3-one, also inhibits cell growth but at higher concentrations than that observed with pentadecan-2-one and that pentadecan-2-o1 at similar concentrations has no effect on cell growth indicates that, for optimal effect, the keto function must be at the 2-position. Inhibition of cell growth by pentadecan-2-one is not unique to HeLa cells, as suggested by the inhibitory effects of this lipid type on the growth of other malignant cell lines of human origin.
通过测量活细胞计数（排除台盼蓝的细胞）以及[¹⁴C]亮氨酸掺入酸沉淀蛋白的情况，评估了在培养介质中加入痕量十五烷-2-酮对HeLa细胞生长的影响。有证据表明，将细胞暴露于痕量的甲基酮（36μg/ml）可有效抑制细胞生长。同时将细胞与长链伯醇十六烷-1-醇一起孵育可缓解这种抑制作用，但与仲醇十五烷-2-醇一起孵育则不能。观察到乙基酮十六烷-3-酮也抑制细胞生长，但浓度高于十五烷-2-酮，且类似浓度的十五烷-2-醇对细胞生长无影响，这表明，为达到最佳效果，酮官能团必须位于2位。十五烷-2-酮对细胞生长的抑制作用并非HeLa细胞所特有，这种脂质类型对其他人类来源的恶性细胞系生长的抑制作用也表明了这一点。
