一种制备高比活放射性磷脂酰-CMP（CDP-二酰基甘油）的快速生物合成方法。
A rapid biosynthetic method for the preparation of radioactive phosphatidyl-CMP(CDP-diacylglycerol) of high specific activity.
作者信息
Eichberg J, Hauser G
出版信息
J Lipid Res. 1978 Aug;19(6):778-83.
PMID:690518
Abstract
A procedrue is described for the preparation of [32P]phosphatidyl-CMP(CDP-diacylglycerol) from rat pineal glands incubated with [32P]orthophosphate and DL-propranolol. The product is 95% radiopure and a high specific acitivity. The yield of liponucleotide is 0.4--0.9 micron Ci/mCi of [32P]orthophosphate in the medium. The same method can also be used for the biosynthesis and purification of [3H]phosphatidyl-CMP when [3H]cytidine is the precursor.
摘要
描述了一种从用[32P]正磷酸盐和DL-普萘洛尔孵育的大鼠松果体中制备[32P]磷脂酰-CMP（CDP-二酰基甘油）的方法。产物的放射性纯度为95%，且具有高比活性。脂质核苷酸的产率为培养基中[32P]正磷酸盐的0.4 - 0.9微居里/毫居里。当[3H]胞苷作为前体时，相同的方法也可用于[3H]磷脂酰-CMP的生物合成和纯化。
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