一种制备高比活放射性磷脂酰-CMP（CDP-二酰基甘油）的快速生物合成方法。
A rapid biosynthetic method for the preparation of radioactive phosphatidyl-CMP(CDP-diacylglycerol) of high specific activity.
作者信息
Eichberg J, Hauser G
出版信息
J Lipid Res. 1978 Aug;19(6):778-83.
PMID:690518
Abstract
A procedrue is described for the preparation of [32P]phosphatidyl-CMP(CDP-diacylglycerol) from rat pineal glands incubated with [32P]orthophosphate and DL-propranolol. The product is 95% radiopure and a high specific acitivity. The yield of liponucleotide is 0.4--0.9 micron Ci/mCi of [32P]orthophosphate in the medium. The same method can also be used for the biosynthesis and purification of [3H]phosphatidyl-CMP when [3H]cytidine is the precursor.
摘要
描述了一种从用[32P]正磷酸盐和DL-普萘洛尔孵育的大鼠松果体中制备[32P]磷脂酰-CMP（CDP-二酰基甘油）的方法。产物的放射性纯度为95%，且具有高比活性。脂质核苷酸的产率为培养基中[32P]正磷酸盐的0.4 - 0.9微居里/毫居里。当[3H]胞苷作为前体时，相同的方法也可用于[3H]磷脂酰-CMP的生物合成和纯化。
相似文献
1
2
The mechanism of modification by propranolol of the metabolism of phosphatidyl-CMP (CDP-diacylglycerol) and other lipids in the rat pineal gland.普萘洛尔对大鼠松果体中磷脂酰-CMP（CDP-二酰基甘油）及其他脂质代谢的修饰机制。
Biochim Biophys Acta. 1979 Apr 27;573(1):90-106. doi: 10.1016/0005-2760(79)90176-0.
3
Accumulation and metabolism of phosphatidyl-CMP1 (CDP-diglyceride) in the pineal gland of the rat.
Adv Exp Med Biol. 1976;72:149-58. doi: 10.1007/978-1-4684-0955-0_14.
4
5
Species pattern of phosphatidic acid, diacylglycerol, CDP-diacylglycerol and phosphatidylglycerol synthesized de novo in rat liver mitochondria.大鼠肝脏线粒体中从头合成的磷脂酸、二酰基甘油、CDP-二酰基甘油和磷脂酰甘油的种类模式。
Biochim Biophys Acta. 1989 Apr 3;1002(2):261-3. doi: 10.1016/0005-2760(89)90296-8.
6
Synthesis of biologically active spin-labelled radioactive cytidine diphosphodiglyceride, a novel probe for biological membranes.具有生物活性的自旋标记放射性胞苷二磷酸甘油酯的合成，一种用于生物膜的新型探针。
Can J Biochem. 1976 Jun;54(6):553-60. doi: 10.1139/o76-081.
7
Synthesis of CDP-diglyceride from phosphatidylinositol and CMP.
Biochem Biophys Res Commun. 1977 Sep 9;78(1):364-71. doi: 10.1016/0006-291x(77)91263-3.
8
Potentiation by lithium of CMP-phosphatidate formation in carbachol-stimulated rat cerebral-cortical slices and its reversal by myo-inositol.锂对卡巴胆碱刺激的大鼠大脑皮层切片中CMP-磷脂酸形成的增强作用及其被肌醇逆转的现象。
Biochem J. 1989 Mar 1;258(2):621-4. doi: 10.1042/bj2580621.
9
The biosynthesis of cytidine diphosphate diacylglycerol in the mammalian cerebral cortex.
Biochem Soc Trans. 1977;5(1):49-51. doi: 10.1042/bst0050049.
10
The CMP-stimulated production of diacylglycerol and CDPdiacylglycerol in neuronal nuclei labelled with radioactive arachidonate.在用放射性花生四烯酸标记的神经元核中，CMP刺激二酰甘油和CDP二酰甘油的产生。
Biochim Biophys Acta. 1985 Jul 9;835(2):221-30. doi: 10.1016/0005-2760(85)90276-0.
Zotero 插件