Hoffman F, Harnisch J P
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1981;215(4):243-8. doi: 10.1007/BF00407663.
In three rabbits a keratophakia as described by Barraquer (1972) was performed on both eyes, in one rabbit it was performed on one eye. In the right eye no cryoprotective agent was used, in the left eye the cryoprotective agent KM 26 was applied. Twelve hours after the operation, the majority of keratocytes in the tissue lens were destroyed. The nuclear chromatin was clumped, the cell membrane was destroyed and cytoplasm showed vacuolar alteration; cell organelles were no longer recognizable. After 24 h hardly any keratocytes were found. All corneae operated on with and without a cryoprotective agent, showed the same morphology. The cell debris was removed by macrophages, which could be found earlier in corneae operated on without cryoprotection. Three weeks after keratophakia no increase in the number of the residual keratocytes had occurred.
对三只兔子的双眼进行了如巴拉克尔（1972年）所描述的角膜镜片植入术，对一只兔子的一只眼睛进行了该手术。右眼未使用冷冻保护剂，左眼应用了冷冻保护剂KM 26。术后12小时，组织晶状体中的大多数角膜细胞被破坏。核染色质凝聚，细胞膜被破坏，细胞质出现空泡样改变；细胞器已无法辨认。24小时后几乎找不到任何角膜细胞。使用和未使用冷冻保护剂进行手术的所有角膜，形态相同。细胞碎片被巨噬细胞清除，在未进行冷冻保护的手术角膜中可更早发现巨噬细胞。角膜镜片植入术后三周，残留角膜细胞数量未增加。
