Hoffmann F
Dev Ophthalmol. 1981;5:41-8. doi: 10.1159/000400936.
With the aid of a thermistor, the temperature decrease in corneal stroma during keratophakia is measured. 1 min after the beginning of the freezing process the temperature is -15 degrees C, 1 min later it is -40 degrees C, and 4 min after the beginning of the freezing process it is -50 degrees C. This temperature course is programmed into the constant rate freezer, in which any number of tissue samples can be frozen at the same time and with a reproducible freezing curve. The preliminary results indicate that under the use of different cryoprotective agents the radioactivity after 35S incorporation is 50-70% of the non-frozen control cornea.
借助热敏电阻，测量了角膜移植术中角膜基质的温度下降情况。冷冻过程开始1分钟后温度为-15℃，1分钟后为-40℃，冷冻过程开始4分钟后为-50℃。该温度变化过程被编入程序控速冷冻仪，在该仪器中可同时冷冻任意数量的组织样本，并具有可重复的冷冻曲线。初步结果表明，在使用不同的冷冻保护剂时，掺入35S后的放射性是未冷冻对照角膜的50%-70%。
