By incubating 6-day post-coitum (p.c.) blastocysts in medium supplemented with either 20 mM-sucrose or 10 mM-KCl it was possible to show that the trophectoderm of the blastocyst prevents the concentration of K in the blastocoele fluid rising to external concentrations. 2. The concentrations of K in the blastocoele fluid are maintained predominantly by leakage of K from the trophoblast cells into the blastocoele and by ouabain-sensitive transport of K into the trophoblast cells from the blastocoele fluid. 3. Exposure of blastocysts to ouabain on the juxtacoelic, but not abcoelic, surface of the trophectoderm may inhibit blastocoele fluid accumulation.
摘要
通过将妊娠6天(p.c.)的囊胚在补充有20 mM - 蔗糖或10 mM - KCl的培养基中培养,可以表明囊胚的滋养外胚层可防止囊胚腔液中K的浓度上升至外部浓度。2. 囊胚腔液中K的浓度主要通过K从滋养层细胞泄漏到囊胚腔以及通过哇巴因敏感的K从囊胚腔液转运到滋养层细胞来维持。3. 将囊胚的滋养外胚层近腔表面而非远腔表面暴露于哇巴因可能会抑制囊胚腔液的积聚。