Benos D J, Biggers J D
J Physiol. 1983 Sep;342:23-33. doi: 10.1113/jphysiol.1983.sp014837.
Influxes of Na+ and Cl- across the trophectodermal epithelium of 6 day post coitum (p.c.) rabbit blastocysts are partially interrelated. This relationship was investigated under conditions in which either the external [Na+] or [Cl-] concentration was varied. Our results indicate that an ouabain-insensitive, furosemide-sensitive, Na+:Cl- co-transport system exists in the 6 and 6.5 day p.c. rabbit embryo, but not in the 5 day p.c. blastocyst. There was no dependence of NaCl uptake on the presence of K+ in the bathing medium. No evidence of Na+/H+ or Cl-/HCO3- exchange systems in the rabbit blastocyst was found. The effects of furosemide, exogenous cyclic AMP, and various exogenous prostaglandins on Na+ and Cl- movements were studied. Cyclic AMP and/or prostaglandin E1 may regulate the operation of this Na+:Cl- co-transport system.
交配后6天（p.c.）的兔胚泡滋养外胚层上皮中Na⁺和Cl⁻的内流部分相关。在改变外部[Na⁺]或[Cl⁻]浓度的条件下研究了这种关系。我们的结果表明，在交配后6天和6.5天的兔胚胎中存在一种哇巴因不敏感、呋塞米敏感的Na⁺:Cl⁻共转运系统，但在交配后5天的胚泡中不存在。NaCl摄取不依赖于浴液中K⁺的存在。未发现兔胚泡中存在Na⁺/H⁺或Cl⁻/HCO₃⁻交换系统。研究了呋塞米、外源性环磷酸腺苷（cAMP）和各种外源性前列腺素对Na⁺和Cl⁻转运的影响。环磷酸腺苷和/或前列腺素E1可能调节这种Na⁺:Cl⁻共转运系统的运作。
