鸡肌球中的乙酰胆碱通道电导的转变温度。
A transition temperature for acetylcholine channel conductance in chick myoballs.
作者信息
Fischbach G D, Lass Y
出版信息
J Physiol. 1978 Jul;280:527-36. doi: 10.1113/jphysiol.1978.sp012399.
Abstract
- The temperature dependence of ACh channel conductance (gamma) and channel open time (tau) was determined by analysing ACh induced membrane current fluctuations in voltage clamped chick myoballs. 2. gamma decreased from 25-30 pmho at 37 degrees C to less than 5 phmo at 10 degrees C. An Arrhenius plot of gamma vs. temperature exhibited a clear break or 'transition temperature' at 20 degrees C. 3. tau increased from 2 msec at 37 degrees C to 16 msec at 10 degrees C. The Arrhenius plot of tau vs. temperature was linear. No transition temperature was detected. 4. Submicellar concentrations of the non-ionic detergent, Triton X-100 reversibly blocked ACh respnses. The effect was all-or-none at the molecular level. 5. These results are consistent with the possibility that the fluidity of membrane lipids in the ACh receptor micro-environment may influence the degree to which the channel can open.
摘要
- 通过分析电压钳制的鸡肌球中的乙酰胆碱（ACh）诱导的膜电流波动，确定了ACh通道电导（γ）和通道开放时间（τ）的温度依赖性。2. γ在37℃时从25 - 30皮西门子降低到10℃时小于5皮西门子。γ与温度的Arrhenius图在20℃时呈现明显的断点或“转变温度”。3. τ从37℃时的2毫秒增加到10℃时的16毫秒。τ与温度的Arrhenius图是线性的。未检测到转变温度。4. 亚胶束浓度的非离子去污剂Triton X - 100可逆地阻断ACh反应。在分子水平上，这种效应是全或无的。5. 这些结果与ACh受体微环境中膜脂流动性可能影响通道开放程度的可能性一致。
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1
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J Physiol. 1972 Aug;224(3):665-99. doi: 10.1113/jphysiol.1972.sp009918.
2
Correlation between electrical activity and splitting of phospholipids by snake venom in the single electroplax.单电鳐中电活动与蛇毒对磷脂的裂解之间的相关性。
J Neurochem. 1972 May;19(5):1251-65. doi: 10.1111/j.1471-4159.1972.tb01451.x.
3
Phase transitions in cells, membranes, and lipids of Escherichia coli. Detection by fluorescent probes, light scattering, and dilatometry.大肠杆菌细胞、细胞膜及脂质中的相变。通过荧光探针、光散射和膨胀测量法进行检测。
Biochemistry. 1973 Jul 3;12(14):2625-34. doi: 10.1021/bi00738a012.
4
Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction.对乙酰胆碱在青蛙神经肌肉接头处产生的终板电流波动进行电压钳分析。
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5
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6
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Proc Natl Acad Sci U S A. 1973 Aug;70(8):2271-5. doi: 10.1073/pnas.70.8.2271.
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