Surovikina M S, Fomina E E
Biull Eksp Biol Med. 1981 May;91(5):623-6.
A method for determining blood plasma kallikrein inhibitors is suggested. It is based on inhibiting the kininogenase activity of kallikrein. The donors' dry plasma devoid of kininases, of other proteolytic enzymes and their inhibitors was used as source and reference of prekallikrein, kallikrein and high-molecular kininogen. Freshly centrifuged plasma was incubated in the presence of unithiol at 37 degrees C in amounts producing a 30-50% inhibition of the kininogenase activity of kallikrein. The values obtained reflected the activity of kallikrein inhibitors and might be of great importance in the study of the physiological role and biochemical properties of kallikrein inhibitors in different animal species, in the diagnosis and prediction of various diseases, as criterion for laboratory control over administering tissue inhibitors of proteolytic enzymes of animal origin.
提出了一种测定血浆激肽释放酶抑制剂的方法。该方法基于抑制激肽释放酶的激肽原酶活性。将不含激肽酶、其他蛋白水解酶及其抑制剂的供体冻干血浆用作前激肽释放酶、激肽释放酶和高分子量激肽原的来源及参照。将新鲜离心的血浆在37℃下于存在二巯基丙醇的情况下进行孵育，二巯基丙醇的用量产生对激肽释放酶激肽原酶活性30%-50%的抑制。所获得的值反映了激肽释放酶抑制剂的活性，在研究不同动物物种中激肽释放酶抑制剂的生理作用和生化特性、诊断和预测各种疾病以及作为对给予动物源蛋白水解酶组织抑制剂进行实验室控制的标准方面可能具有重要意义。
