Surovikina M S, Maslikova G V, Fomina E E
Biull Eksp Biol Med. 1983 May;95(5):106-9.
The discrepancies between kininogenase, BAEE-esterase and BAPNA-amidase activity in plasma kallikrein were shown in experiments in vitro. The same discrepancies were detected during examination of children aged under 3 years suffering from bronchopulmonary diseases (acute and lingering pneumonia, bronchial asthma). In these children, prekallikrein, kallikrein and kallikrein inhibitor were determined at a time by the 3 methods (esterase, test-tube chromatographic and kininogenase ones). It has been shown that the kininogenesis intensity could be assessed objectively only in the measurement of the kininogenase activity of kallikrein contained by the whole plasma or its fractions. The esterase technique characterizes total factors of contact activation and does not reflect the activity of kallikrein, prekallikrein and kallikrein inhibitor. The test-tube chromatographic method might be regarded as objective only in the measurement of the kininogenase activity of the fractions that contain kallikrein or prekallikrein.
体外实验显示了血浆激肽释放酶中激肽原酶、BAEE酯酶和BAPNA酰胺酶活性之间的差异。在对3岁以下患有支气管肺部疾病（急性和迁延性肺炎、支气管哮喘）的儿童进行检查时也发现了同样的差异。通过三种方法（酯酶法、试管色谱法和激肽原酶法）同时测定了这些儿童的前激肽释放酶、激肽释放酶和激肽释放酶抑制剂。结果表明，只有通过测量全血浆或其组分中所含激肽释放酶的激肽原酶活性，才能客观地评估激肽生成强度。酯酶技术表征了接触激活的总因素，并未反映激肽释放酶、前激肽释放酶和激肽释放酶抑制剂的活性。试管色谱法只有在测量含有激肽释放酶或前激肽释放酶的组分的激肽原酶活性时才可视为客观。
