Feichtinger W, Szalay S, Kemeter P, Beck A, Janisch H
Geburtshilfe Frauenheilkd. 1981 Jul;41(7):482-9. doi: 10.1055/s-2008-1037288.
Methods and Results of human in vitro fertilisation at the 2nd Department of Obstetrics and Gynaecology, University of Vienna are reported. Applying preparations of media and culture techniques according to Edwards et al. no satisfactory results could be achieved: only 3 out of 11 preovulatory oocytes were fertilised, no cleavages occurred. Using modified Ham's F 10 medium according to Lopata et al. fertilisation could be observed in 60% as well as regular cleavages in 40%. 5 Embryotransfers have been performed in the 8 cell or in the 16 cell stage respectively. No implantation occurred in 4 of these, in one case evidence for a transient implantation could be proved by an increased activity of SP1 and HCG beta. In this case obviously postimplantation death occurred resulting in a bleeding 16 days after oocyte recovery. Possible reasons for failures in fertilisation and cleavage as well as possibilities for improving the rate of reimplantation are discussed.
本文报告了维也纳大学第二妇产科人类体外受精的方法与结果。按照爱德华兹等人的方法应用培养基制剂和培养技术，未取得满意结果：11个排卵前卵母细胞中仅有3个受精，且未发生分裂。根据洛帕塔等人的方法使用改良的哈姆F10培养基，60%的卵母细胞实现了受精，40%出现了正常分裂。分别在8细胞期或16细胞期进行了5次胚胎移植。其中4次未发生着床，1例通过SP1和HCGβ活性增加证明有短暂着床迹象。在此病例中，明显发生了着床后死亡，导致在卵母细胞回收后16天出现出血。文中讨论了受精和分裂失败的可能原因以及提高再植入率的可能性。
