Seleznev A S, Bykov A S
Antibiotiki. 1981 Jul;26(7):514-9.
Morphological changes in phagocytized cells of Staph. aureus due to methicillin were studied with electron microscopy of ultrathin sections. Peritoneal macrophages of mice obtained by excitation of the abdominal cavity with medium No. 199 followed by infection with 1-milliard staphylococcal suspension were tested. Methicillin was administered in single doses of 2.5, 5, 25 and 50 mg/kg. The doses of 2.5 and 5 mg/kg induced specific changes in some cells. First of all the division septa were affected. After that the cell walls became thinner the cells increased in size, the cytoplasm became electronically less solid. No lysed cells were detected. The use of the doses of 25 and 50 mg/kg entailed polymorphism of the damages. Perforations formed in the bacterial cell wall. The cytoplasm acquired a characteristic granular structure evident of the cell death. With an increase in the dose from 25 to 50 mg/kg no rise in the level of the morphological changes in the bacterial cells was noted. Determination of methicillin concentration in the peritoneal exudate showed that it was 2--4 times higher depending on the dose than the MIC in vitro.
通过超薄切片电子显微镜研究了甲氧西林对金黄色葡萄球菌吞噬细胞形态变化的影响。对通过用199号培养基激发腹腔然后感染10亿个葡萄球菌悬液获得的小鼠腹腔巨噬细胞进行了测试。甲氧西林以2.5、5、25和50mg/kg的单剂量给药。2.5和5mg/kg的剂量在一些细胞中引起了特定变化。首先，分裂隔膜受到影响。之后细胞壁变薄，细胞体积增大，细胞质在电子显微镜下变得不那么致密。未检测到裂解细胞。使用25和50mg/kg的剂量导致损伤的多态性。细菌细胞壁形成穿孔。细胞质获得了明显表明细胞死亡的特征性颗粒结构。随着剂量从25mg/kg增加到50mg/kg，未观察到细菌细胞形态变化水平的升高。测定腹腔渗出液中甲氧西林的浓度表明，根据剂量不同，其比体外最低抑菌浓度（MIC）高2至4倍。
