Corthorn J, Roblero J
Hypertension. 1981 Nov-Dec;3(6 Pt 2):II-39-41. doi: 10.1161/01.hyp.3.6_pt_2.ii-39.
Rat isolated kidneys were perfused for 60 minutes with a modified Krebs-Henseleit bicarbonate solution. Perfusate and urine samples showed kininogenase activity (active kallikrein) which could be enhanced by activation with trypsin (activatable kallikrein). Identification of the kininogenase activity generated by trypsin was made with rat renal kallikrein antiserum, aprotinin, lima bean trypsin inhibitor (LBTI), soybean trypsin inhibitor (SBTI), and ovomucoid. A sample of perfusate was partially purified through DEAD-Sephacel chromatography. Intraarterial injection of this fraction decreased blood pressure in the perfused hind limb of a rat.
用改良的克雷布斯 - 亨泽莱特碳酸氢盐溶液对大鼠离体肾脏进行60分钟灌注。灌注液和尿液样本显示出激肽原酶活性（活性激肽释放酶），用胰蛋白酶激活（可激活激肽释放酶）可增强该活性。用大鼠肾激肽释放酶抗血清、抑肽酶、利马豆胰蛋白酶抑制剂（LBTI）、大豆胰蛋白酶抑制剂（SBTI）和卵类粘蛋白对胰蛋白酶产生的激肽原酶活性进行鉴定。通过DEAE - 葡聚糖凝胶层析对一份灌注液样本进行部分纯化。向大鼠灌注后肢动脉内注射该组分可降低血压。
