Morosco G J, Nightingale T E, Frasinel C, Goeringer G C
J Toxicol Environ Health. 1981 Jul-Aug;8(1-2):89-94. doi: 10.1080/15287398109530052.
Levels of elastase activity in homogenates of pancreas from beagle dogs exposed to cigarette smoke on a daily basis for 600 d were previously reported. Elastase activity in pancreatic homogenates from animals exposed to smoke from high-nicotine cigarettes was significantly greater than in sham-exposed controls or in animals smoking low-nicotine cigarettes. In the study described here, dogs were exposed on an acute basis to smoke from high-nicotine and from nicotine-free cigarettes. Anesthetized animals were prepared by laparotomy, duodenotomy, and pancreatic duct cannulation. Pancreatic fluid was collected and preexposure elastase levels were determined. Samples were then taken during exposure to smoke from cigarettes of both types. There was no measurable elastase activity in the baseline samples. Similarly, no activity was found in fluids collected before and during exposure to smoke from nicotine-free cigarettes. Enzyme activity was detected, however, in samples collected during exposure to smoke from high-nicotine cigarettes once blood levels of nicotine reached 50-70 ng/ml. The results suggest that cigarette smoke may provoke activation of elastase and indicate the need for further study of any association of cigarette smoking with pancreatic disease.
此前曾报道过，每天接触香烟烟雾600天的比格犬胰腺匀浆中的弹性蛋白酶活性水平。接触高尼古丁香烟烟雾的动物胰腺匀浆中的弹性蛋白酶活性显著高于假暴露对照组或吸低尼古丁香烟的动物。在本研究中，犬类被急性暴露于高尼古丁香烟和无尼古丁香烟的烟雾中。通过剖腹术、十二指肠切开术和胰管插管对麻醉动物进行准备。收集胰液并测定暴露前的弹性蛋白酶水平。然后在暴露于两种类型香烟烟雾期间采集样本。基线样本中未检测到可测量的弹性蛋白酶活性。同样，在暴露于无尼古丁香烟烟雾之前和期间收集的液体中也未发现活性。然而，一旦血液中的尼古丁水平达到50 - 70 ng/ml，在暴露于高尼古丁香烟烟雾期间收集的样本中检测到了酶活性。结果表明，香烟烟雾可能会引发弹性蛋白酶的激活，并表明有必要进一步研究吸烟与胰腺疾病的任何关联。
