Zolovick A J, Rossi J, Davies R F, Panksepp J
Eur J Pharmacol. 1982 Feb 5;77(4):265-71. doi: 10.1016/0014-2999(82)90128-5.
A pharmacological procedure which initially depletes noradrenaline (NA), dopamine (DA) and 5-hydroxytryptamine (5-HT) but permits repletion of DA and 5-HT was used to evaluate the role of NA in feeding behavior and intracranial self-stimulation behavior. The rapid-onset 'reserpine-like' vesicular depletion drug RO 4-1284 reduced NA and 5-HT 99% and DA 90% in rat forebrain within 1 h after administration with complete repletion of all amines occurring within 6 to 12 h. Treatment with the dopamine-beta-hydroxylase inhibitor FLA-63 significantly reduced NA (maximum depletion 42%) but not DA or 5-HT over the 12 h period of evaluation. The two drugs together produced a specific depletion of NA. Forebrain levels of NA in subjects pretreated with FLA-63 then given RO 4-1284 0.5 h later were reduced to 2% of control values for 8 h while vesicular stores of DA and 5-HT were repleted 77% and 93%, respectively, within 8 h after administration. Selective depletion of NA, in this manner, reduced deprivation induced food intake and lateral hypothalamic self-stimulation.
一种药理学方法最初会耗尽去甲肾上腺素（NA）、多巴胺（DA）和5-羟色胺（5-HT），但允许DA和5-HT重新补充，该方法用于评估NA在摄食行为和颅内自我刺激行为中的作用。起效迅速的“利血平样”囊泡耗竭药物RO 4-1284在给药后1小时内使大鼠前脑内的NA和5-HT减少99%，DA减少90%，所有胺类在6至12小时内完全重新补充。在12小时的评估期内，用多巴胺-β-羟化酶抑制剂FLA-63治疗可显著降低NA（最大耗竭42%），但不降低DA或5-HT。这两种药物共同作用产生了NA的特异性耗竭。先用FLA-63预处理，然后在0.5小时后给予RO 4-1284的受试者，其前脑NA水平在8小时内降至对照值的2%，而DA和5-HT的囊泡储存分别在给药后8小时内重新补充了77%和93%。以这种方式选择性耗尽NA可减少剥夺诱导的食物摄入量和下丘脑外侧自我刺激。
