Goncharov N I
Ukr Biokhim Zh (1978). 1982 Mar-Apr;54(2):204-7.
The aminoacylation of tRNA was investigated with respect to protein synthesis in the rat liver. No correlation was found between the 85-90% inhibition of protein synthesis 2 h after cycloheximide injection and aminoacylation level of some tRNAs both in vivo and in vitro. A decrease in aminoacylation (28%) was established only for lysine. During the recovery phase of protein synthesis 12 and 24 h after cycloheximide treatment the aminoacylation maximal level of mixture with 14C amino acids, 14C leucine, 14C glutamic acid was unchanged.
针对大鼠肝脏中的蛋白质合成，对tRNA的氨酰化作用进行了研究。在注射环己酰亚胺2小时后，蛋白质合成受到85 - 90%的抑制，但在体内和体外实验中，均未发现这种抑制与某些tRNA的氨酰化水平之间存在相关性。仅发现赖氨酸的氨酰化作用有所降低（28%）。在环己酰亚胺处理后12小时和24小时的蛋白质合成恢复阶段，与14C氨基酸、14C亮氨酸、14C谷氨酸混合的氨酰化最大水平未发生变化。
