Keith G
Biochimie. 1986 Mar;68(2):325-7. doi: 10.1016/s0300-9084(86)80030-x.
The primary structure of threonine tRNA (anticodon I-G-U) from bovine liver has been determined using 32P postlabeling methods. Its nucleotide sequence is pG-G-C-C-C-U-G-U-m1G-m2G-C-U-A-G-C-D-G-G-D-C-A-A-A-G-C-m2(2)G-C-C- U-G-U-m3 C-U-I-G-U-t6A-A-A-C-A-G-G-A-G-A-D-m5C-C-U-G-G-G-U-psi-C-G-m1 A-A-U-C-C-C-A-G-C-G-G-G-G-C-m5C-U-C-C-A. The anticodon I-G-U, according to the wobble hypothesis, is expected to recognize A-C-U, A-C-C and A-C-A, 3 out of the 4 codons for threonine. However, if the original wobble hypothesis, as it has been shown recently for several yeasts and a fungus, is only restricted to U and C in the ox, it would recognize only 2 codons: A-C-C and A-C-U.
利用32P后标记法确定了来自牛肝脏的苏氨酸tRNA（反密码子I-G-U）的一级结构。其核苷酸序列为pG-G-C-C-C-U-G-U-m1G-m2G-C-U-A-G-C-D-G-G-D-C-A-A-A-G-C-m2(2)G-C-C-U-G-U-m3C-U-I-G-U-t6A-A-A-C-A-G-G-A-G-A-D-m5C-C-U-G-G-G-U-ψ-C-G-m1A-A-U-C-C-C-A-G-C-G-G-G-G-C-m5C-U-C-C-A。根据摆动假说，反密码子I-G-U预计能识别4个苏氨酸密码子中的3个：A-C-U、A-C-C和A-C-A。然而，正如最近在几种酵母和一种真菌中所显示的那样，如果原始的摆动假说在牛中仅局限于U和C，那么它将只能识别2个密码子：A-C-C和A-C-U。
