生物碱与植物转运核糖核酸的相互作用。鹰爪豆碱对羽扇豆精氨酰 - tRNA形成的影响。

Interaction of alkaloids with plant transfer ribonucleic acids. Effect of sparteine on lupin arginyl-tRNA formation.

作者信息

Zwierzyński T, Joachimiak A, Barciszewska M, Kulińska K, Barciszewski J

出版信息

Chem Biol Interact. 1982 Oct;42(1):107-16. doi: 10.1016/0009-2797(82)90146-6.

Abstract

The effect of the alkaloid sparteine on arginyl-tRNA formation was studied. It was demonstrated that sparteine sulfate in the concentration range 10-60 mM inhibits the charging reaction when amino acid, ATP and tRNA are used as variable substrates. The mode of action is different for all pattern of inhibition for all varied substrates is generally uncompetitive. A pattern of inhibition for all varied substrates is generally uncompetitive. A non-competitive mechanism for amino acid and tRNA was observed at low sparteine concentration, but in the case of ATP it is also uncompetitive.

摘要

研究了生物碱金雀花碱对精氨酰 - tRNA形成的影响。结果表明，当氨基酸、ATP和tRNA用作可变底物时，浓度范围为10 - 60 mM的硫酸金雀花碱会抑制氨酰化反应。对于所有不同底物的抑制模式，其作用方式不同，一般为非竞争性抑制。对于所有不同底物的抑制模式通常都是非竞争性的。在低金雀花碱浓度下，观察到氨基酸和tRNA的抑制机制为非竞争性，但对于ATP而言，其抑制机制也是非竞争性的。