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[从临床样本中鉴定出46株酵母:形态学标准、生化及免疫学检测]

[Identification of 46 yeasts from a clinical source: morphological criteria, biochemical and immunological tests].

作者信息

Riviera L, Poggiolini D

出版信息

Boll Ist Sieroter Milan. 1982;61(6):505-9.

PMID:6927225
Abstract

From clinical specimens (urine, pharyngeal and vaginal exudates, sputum and skin) 46 yeasts have been identified. The cultures have been divided into 3 groups, according to their morphological characters: unicellular, yeasts having hyphae, pseudohyphae, blastospores and yeasts producing also arthrospores. Tests for fermentation and assimilation of carbohydrates have been employed for S.cerevisiae, T.cutaneum and T.glabrata. By the agglutination tests with monospecific antisera, 40 strains of Candida spp. and T.glabrata are identified; this method was able to differentiate 2 C.stellatoidea from C.albicans group B and 4 C.tropicalis from C.albicans group A. The immunologic method proved highly valuable and practical.

摘要

从临床标本(尿液、咽部分泌物、阴道分泌物、痰液和皮肤)中已鉴定出46株酵母。根据其形态特征,培养物被分为3组:单细胞酵母、具有菌丝、假菌丝、芽生孢子的酵母以及还产生节孢子的酵母。已对酿酒酵母、光滑假丝酵母和白吉利丝孢酵母进行了碳水化合物发酵和同化试验。通过用单特异性抗血清进行凝集试验,鉴定出40株念珠菌属菌株和白吉利丝孢酵母;该方法能够区分B组白色念珠菌中的2株星状念珠菌和A组白色念珠菌中的4株热带念珠菌。免疫方法被证明具有很高的价值和实用性。

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