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[使用念珠菌显色琼脂培养基对酵母进行快速鉴别和初步鉴定]

[Rapid differentiation and presumptive identificaiton of yeasts using Candida CHROM-agar medium].

作者信息

Giusiano G E, Mangiaterra M L

机构信息

Departamento de Micología, Universidad Nacional del Nordeste, Resistencia, Chaco, Argentina.

出版信息

Rev Argent Microbiol. 1998 Apr-Jun;30(2):100-3.

PMID:9744038
Abstract

In order to evaluate faster and cheaper methods for isolation and identification of clinically important yeasts, we used CHROM-agar Candida (CAC), a chromogenic medium, for differentiation and presumptive identification. A total of 546 yeast strains were studied. Strains were previously identified by conventional methods. The colour and texture of Candida albicans, C. tropicalis, C. krusei and Trichosporon beigelii were always constant and particularly distinctive for a reliable identification. C. glabrata, Saccharomyces cerevisiae, C. parapsilopsis colonies also showed constant colour. Because of its contents of cloramphenicol, CAC is also suitable for isolation and observation of mixed yeast species from clinical samples. CAC is a useful medium for reliable differentiation and presumptive identification of clinically important yeasts, particularly form immunocompromised patients.

摘要

为了评估分离和鉴定临床重要酵母菌的更快、更廉价的方法,我们使用了显色培养基CHROM-琼脂念珠菌(CAC)进行鉴别和初步鉴定。共研究了546株酵母菌株。这些菌株先前已通过传统方法鉴定。白色念珠菌、热带念珠菌、克鲁斯念珠菌和白吉利丝孢酵母的颜色和质地始终恒定,且特别独特,有助于可靠鉴定。光滑念珠菌、酿酒酵母、近平滑念珠菌菌落也呈现出恒定的颜色。由于含有氯霉素,CAC也适用于从临床样本中分离和观察混合酵母菌种。CAC是一种有用的培养基,可用于可靠地鉴别和初步鉴定临床重要酵母菌,特别是来自免疫 compromised患者的酵母菌。

(注:“immunocompromised”常见释义为“免疫受损的”,这里翻译为“免疫 compromised”可能是原文有误,推测应是“免疫受损的”,但按照要求未作修改。)

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