Dodd D E, Faiman M D
Toxicology. 1982;24(3-4):325-33. doi: 10.1016/0300-483x(82)90014-2.
Experiments were done in vivo to determine the effect of oxygen at high pressure (OHP) on NAD+ and NADH in mouse brain cortex. A 7% decrease (N.S.) in NADH was found in brain cortex from mice exposed to 6 atm of 100% oxygen for 8 min, while a 20% decrease (P less than 0.01) in cortical NADH, when compared to controls, occurred when mice were exposed to this oxygen pressure for either 16 min or 48 min. A 20% decrease (P less than 0.05) in cortical NADH was also observed in mice which had been killed during hyperactivity (a state preceding convulsions), at seizure onset, or 10 s post-convulsions. No measurable change in cortical NAD+ was observed at any of these oxygen exposure times or stages of toxicity. When mice were exposed to either 3.5 atm or 6 atm of oxygen for 16 min, a statistically significant decrease in cortical NADH (P less than 0.01) coupled with an increase in the NAD+/NADH ratio was found only at 3.5 atm and 6 atm, and not at 1 atm. The decrease in cortical NADH and increase in the NAD+/NADH ratio were reversed when mice were decompressed and exposed to air for 30 min. Disulfiram, a drug found to delay the onset of oxygen seizures, did not prevent the oxygen-induced decrease in cerebral NADH or increase in the NAD+/NADH ratio. The decrease in cortical NADH in mice exposed to OHP did not correlate with the onset of oxygen-induced convulsions.
进行了体内实验，以确定高压氧（OHP）对小鼠大脑皮质中NAD⁺和NADH的影响。暴露于6个大气压的纯氧中8分钟的小鼠，其大脑皮质中的NADH下降了7%（无统计学意义）；而暴露于该氧压16分钟或48分钟的小鼠，其皮质NADH与对照组相比下降了20%（P<0.01）。在活动亢进期（惊厥前状态）、惊厥发作时或惊厥发作后10秒处死的小鼠中，也观察到皮质NADH下降了20%（P<0.05）。在这些氧暴露时间或毒性阶段的任何时候，均未观察到皮质NAD⁺有可测量的变化。当小鼠暴露于3.5个大气压或6个大气压的氧气中16分钟时，仅在3.5个大气压和6个大气压下发现皮质NADH有统计学显著下降（P<0.01），同时NAD⁺/NADH比值增加，而在1个大气压下未出现这种情况。当小鼠减压并暴露于空气中30分钟后，皮质NADH的下降和NAD⁺/NADH比值的增加得以逆转。双硫仑是一种可延迟氧惊厥发作的药物，但它并不能阻止氧诱导的脑NADH下降或NAD⁺/NADH比值增加。暴露于高压氧的小鼠皮质NADH下降与氧诱导惊厥的发作无关。
